Veterinary and Animal Sciences
Scientific Journals Home Page
Turkish Journal of Veterinary and Animal Sciences
Demonstration of Infectious Bovine Rhinotracheitis (IBR) Virus Antigens by
Hakan BULUT, Yusuf BOLAT, Aykut ÖZDARENDELİ
Fırat Üniversitesi, Veteriner Fakültesi, Viroloji Anabilim Dalı, Elazığ-TÜRKİYE
Mehmet Z. DOYMAZ
Fırat Üniversitesi, Tip Fakültesi, Mikrobiyoloji Anabilim Dalı, Elazığ-TÜRKİYE
S. İsmet GÜRHAN
Şap Enstitüsü, Ankara-TÜRKİYE
Abtract: In this study, cellular localization of IBR-IPV (Infectious Bovine Rhinotracheitis-Infectious Pustular Vulvovaginitis) virus anti-gens
at various times of infection was studied. For this purpose, MDBK cells wre infected with IBR-Colorado strain and cellular dis-tribution
of viral antigens were followed at early and late periods of infetction. Immunohistochemical staining of virus antigens were
performed by using IBR-Colorado immune Balb/C mouse serum and horse radish peroxidase-conjugated goat anti-mouse immuno-globins
as primary and secondary antibodies, respectively. Until the third hour of infection, viral antigens were confined to the nucle-us
of infected cells, however, at later times, viral antigens were distributed to both nucleus and cytoplasm. As the time period of
infection increased, the amount of viral antifens detected became abundant. At those period, typical cytopathic changes of IBR-IPV
infection such as swelling of the cells and distention of cytoplasm were noticed in infected cells.
Key Words: IBR, ELISA, Immunoperoxidase
Turk. J. Vet. Anim. Sci. 1998; 22: 267-272.
Full text: pdf
Other articles published in the same issue: Turk. J. Vet. Anim. Sci.,vol.22,iss.3.