Veterinary and Animal Sciences
Turkish Journal of Veterinary and Animal SciencesInfluence of cyclosporine A and quercetine on MIC values of danofloxacin mesylate in Escherichia coli strains isolated from poultry
1Department of Veterinary Microbiology, Infectious and Parasitic Diseases, Section of Microbiology,
Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, BULGARIA
2Department of Pharmacology, Veterinary Physiology and Physiological Chemistry, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, BULGARIA
Abstract: A reference strain Escherichia coli ATCC 25922, sensitive and resistant strains of E. coli, isolated from chickens with colibacillosis were tested. The aim of the present study was to determine the effect of modulators of ABC efflux transporters in eukaryotic cells cyclosporine A and quercetine on MIC values of danofloxacin mesylate. MIC values of danofloxacine mesylate against the chosen strains were determined with or without modulators at concentrations of 25 m M, 5 m M, 1 m M, and 0.2 m M by macro broth dilution method. Results show that in the presence of cyclosporine A, MIC values decreased from 2-fold to 16-fold in the investigated strains of E. coli. Combination of danofloxacin mesylate and quercetine resulted in an increase of MIC values. The observed changes in the values of MICs suggest participation of ATP-dependent efflux of danofloxacin mesylate from bacterial cells.
Key Words: Danofloxacin mesylate, cyclosporine A, quercetine, Escherichia coli
Turk. J. Vet. Anim. Sci. 2009; 33: 247-251.
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Other articles published in the same issue: Turk. J. Vet. Anim. Sci.,vol.33,iss.3.