Turkish Journal
of
Agriculture and Forestry
agric@tubitak.gov.tr
Turkish Journal of Agriculture and Forestry
Heterologous Expression of Genes in the Yeast Saccharomyces cerevisiaeÇukurova University,Agricultural Faculty,Department of Animal Science,Adana -TURKEY
Abstract: Both an a-amylase gene of Bacillus subtilis RSKK246 and a gene encoding b-glucanase from B.subtilis RSKK243 were cloned and expressed in both E.coli XL1-Blue MRF and B.subtilis YB886 by using the vectors pUC18 and pUB110 respectively. These genes were also cloned into the E.coli-yeast shuttle vectors pRS406 and pRS416 for transfer into the yeast Saccharomyces cerevisiae.These constructs carrying a -amylase and a -glucanase,genes which were cloned into the pRS406 vector,were expressed by integration into the yeast chromosome.These genes were also expressed in the yeast by autonomous replication of the ARS/CEN plasmid pRS416.This work demonstrates that genes belonging to B.subtilis can replicate in both E.coli and in the yeast S. cerevisiae.
Key Words: a-amylase,b-glucanase,Saccharomyces cerevisiae,Bacillus subtilis,gene cloning
Turk. J. Agric. For., 25, (2001), 45-49.
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