Turkish Journal of Agriculture and Forestry

Research on antifungal and inhibitory effects of DL-limonene on some yeasts
Mustafa Ümit ÜNAL¹, Filiz UÇAN¹, Aysun ŞENER¹, Sadık DİNÇER²
¹Department of Food Engineering, Faculty of Agriculture, Çukurova University, Adana - TURKEY
²Department of Biology, Faculty of Science and Letters, Çukurova University, Adana - TURKEY

Abstract: This study was undertaken to investigate the antifungal effect of DL-limonene on yeasts using disk diffusion and the minimum inhibitory concentration (MIC). The effect of DL-limonene on ethyl alcohol fermentation by Saccharomyces cerevisiae was also investigated. According to disk diffusion results, the development of an inhibition zone was observed in all microorganisms tested. The inhibition zones were larger than those occurring with the Fungizone antibiotic used as the control. Inhibition zone diameters increased with increasing amounts of DL-limonene. MIC results varied between 500 and 4000 µg mL^-1 depending on the yeast strain. DL-limonene at a concentration of 0.20% (w v^-1) inhibited cell growth, ethanol formation, and sugar utilization by S. cerevisiae.

Key words: Antifungal effect, DL-limonene, fermentation, yeasts

Turk. J. Agric. For., 36, (2012), 576-582.
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Other articles published in the same issue: Turk. J. Agric. For.,vol.36,iss.5.