Turkish Journal
of
Agriculture and Forestry
agric@tubitak.gov.tr
Turkish Journal of Agriculture and Forestry
Molecular characterization and genetic diversity analysis of citrus cultivars by RAPD markers1National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, P. R. CHINA
2National Research Center on Plant Biotechnology, Indian Agricultural Research Institute, 110012, New Delhi, INDIA
3Department of Molecular Biology and Biotechnology, Department of Biochemistry
CCS Haryana Agricultural University, Hisar, Haryana 125004, INDIA
Abstract: Random Amplified Polymorphic DNA (RAPD) markers were used to evaluate genetic similarity and inter-relationship among 18 citrus cultivars, including 13 species and 5 hybrids. Out of 40 decamer primers screened, 25 were selected which produced 250 markers; of which 231 were polymorphic and some species or cultivar specific RAPD markers. The Jaccard coefficient was used to calculate the genetic similarity. UPGMA was used to generate the dendrogram which clearly separated Jatti-Khatti from all major clusters at a similarity coefficient of 0.61. The average genetic similarity value observed across all the genotypes was 0.63, with the 2 sweet orange cultivars, Jaffa and Blood red, showing maximum similarity (82%). The Jatti-Khatti and King Mandarin were found to be genetically most diverse. The genetic variation between cultivars was quite high and revealed their different origins.
Key words: Citrus, RAPD, UPGMA, Dendrogram, Genetic diversity, PCR
Turk. J. Agric. For., 33, (2009), 375-384.
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Other articles published in the same issue: Turk. J. Agric. For.,vol.33,iss.4.