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Turkish Journal of Medical Sciences

Typing of Methicillin-Resistant Staphylococcus aureus by Ribosome Spacer and Arbitrarily Primed Polymerase Chain Reaction

Tamer ESSAWI1, Kamel ADWAN2, Nael ABU-HASSAN2, Galeb ADWAN2, Ahmed SALEH2
1Master Program in Clinical Laboratory Sciences, Birzeit University, Palestine
2Department of Biological Sciences, An-Najah N. University, Palestine

Abstract: Thirty-five methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates were collected from 3 hospitals in the northern and southern parts of Palestine between February and May 1998. These isolates were typed by ribosome spacer PCR (RS-PCR) and arbitrarily primed PCR (AP-PCR). RS-PCR generated 9 different genotypes. The use of AP-PCR provided a high resolution typing method and allowed us to define 11 different clusters. Three major clusters, however, based on the combination of both typing methods, spread throughout the neonatal and intensive care units of Rafidya Hospital during the entire period.

Key Words: Staphylococcus aureus, epidemiology, polymerase chain reaction

Turk J Med Sci 2004; 34(1): 5-9.
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Other articles published in the same issue: Turk J Med Sci,vol.34,iss.1.