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Turkish Journal of Botany

Insertion of an Antimicrobial Gene into Agrobacterium and its Further Use in Transforming Tobacco

Ekrem GÜREL
Abant Izzet Baysal University, Faculty of Science and Arts, Department of Biology, 14280 Bolu - TURKEY

Abstract: In the present work, we first describe the transfer of a novel gene, designated 5W, into the LBA4404 Agrobacterium tumefaciens strain using pPCV91 plasmid through a triparental mating system. The 5W gene was recently isolated from macadamia, a native nut tree species of Australia, and has been shown to have an antimicrobial effect against certain fungi in vitro. Secondly, this gene was tranferred into tobacco (Nicotiana tabacum cv. Xhantii) using leaf explants to develop a system which could then be used for transferring this antimicrobial peptide into sunflower cultivars.

Key Words: Macademia, tobacco, triparental mating, gene transfer, leaf explants

Turk. J. Bot., 25, (2001), 169-175.
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Other articles published in the same issue: Turk. J. Bot.,vol.25,iss.4.