Turkish Journal of BiologyEffect of a-Keto Acids Against H2O2- and NaN3-Induced Mutagenesis in Different Strains of Salmonella Typhimurium
Department of Molecular Biology, Faculty of Science, Hacettepe University, Ankara - TURKEY
Abstract: The protective effects of a-keto acids on the mutagenicity of H2O2 and NaN3 was investigated in Salmonella typhimurium TA 98, TA 100, TA 102. H2O2 (340 µg/plate) was mutagenic to the strains TA 98 and TA 102 while NaN3 (1.5 µg/plate) was mutagenic only to TA100. The extents of protective effect of pyruvate (220-440 µg/plate) and a-keto glutarate (335-600 µg/plate) against H2O2 were observed to be 37%- 65% in TA 98 and TA 102. a-keto acids were ineffective against NaN3 induced mutagenicity in TA 100. H2O2 (340 µg/plate), in the presence of NaN3 (1.5 µg/plate) and a-keto acids (330-500 µg/plate), was found to be nonmutagenic to the strain TA102. This cross-adaptive response might have been due to the intactness of the DNA repair system in TA 102. The above combination of H2O2, NaN3 and a-keto acids were found to be cytotoxic in TA 98 and TA 100, possibly because these strains carry uvr B mutation causing defect in their DNA repair system.
Key Words: a-keto acids, H2O2, NaN3, Ames test system, mutagenicity, antimutagenicity
Turk. J. Biol., 29, (2005), 161-166.
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Other articles published in the same issue: Turk. J. Biol.,vol.29,iss.3.