Turkish Journal of BiologyAntimicrobial Effects of Ocimum basilicum (Labiatae) Extract
Hatice ÖĞÜTCÜ2, Fikrettin ŞAHİN1, İsa KARAMAN1
1Biotechnology Application and Research Center, Atatürk University, 25240, Erzurum - TURKEY
2Department of Biology, Faculty of Arts and Science, Atatürk University, 25240, Erzurum - TURKEY
Abstract: Ethanol, methanol, and hexane extracts from Ocimum basilicum Labiatae (sweet basil) were investigated for their invitro antimicrobial properties. A total of 146 microbial organisms belonging to 55 bacteria, and four fungi, and a yeast species were studied using a disk-diffusion and minimal inhibition concentration (MIC) method. The result showed that none of the three extracts tested have antifungal activities, but anticandidal and antibacterial effects. Both the hexane and methanol extracts, but not the ethanol extracts, inhibited three isolates out of 23 strains of Candida albicans studied. All three extract of O. basilicum were different in terms of their antibacterial activities. The hexane extract showed a stronger and broader spectrum of antibacterial activity, followed by the methanol and ethanol extracts, which inhibited 10, 9 and 6% of the 146 bacterial strains tested, respectively. The minimal inhibition zones (MIC) of the hexane, methanol, and ethanol extracts ranged from 125 to 250 µl/ml, 62.50 to 500 µl/ml, and 125 to 250 µl/ml, respectively.
Key Words: Ocimum basilicum Labiatae., antimicrobial activity, medicinal plant
Turk. J. Biol., 29, (2005), 155-160.
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Other articles published in the same issue: Turk. J. Biol.,vol.29,iss.3.