Turkish Journal of BiologyEffects of Carbon Sources and Various Chemicals on the Production of a Novel Amylase from a Thermophilic Bacillus sp. K-12
1Kahramanmaraş Sütçü İmam University, Faculty of Arts and Sciences, Department of Biology, Kahramanmaraş - TURKEY
2Çukurova University, Faculty of Arts and Sciences, Department of Biology, Adana - TURKEY
Abstract: The amylase producer thermophilic Bacillus sp. K-12 was isolated from soil samples from Zeytinli hot spring in Kahramanmaraş. Enzyme synthesis occurred at 20-55 ºC with an optimum of 42 ºC. There was a slight variation in amylase synthesis within the pH range 4.5-10.5. Effects of various carbon sources and chemicals on a-amylase production were examined and maximum a-amylase production was obtained in a medium containing 1% starch in 60 h. MnSO4, ZnSO4 and EDTA inhibited a-amylase production of Bacillus sp. K-12.
Key Words: Thermostable, Amylase, Bacillus
Turk. J. Biol., 29, (2005), 99-103.
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